{"id":338074,"date":"2024-10-19T23:38:58","date_gmt":"2024-10-19T23:38:58","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-12393-22013\/"},"modified":"2024-10-25T22:44:51","modified_gmt":"2024-10-25T22:44:51","slug":"bs-en-12393-22013","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-12393-22013\/","title":{"rendered":"BS EN 12393-2:2013"},"content":{"rendered":"<p>This European Standard specifies methods for the extraction and clean-up of food samples of plant origin for quantitative determination of pesticide residues.<\/p>\n<p>Different solvents can be used for this purpose. These pesticide residues are generally associated with other co-extracted compounds which would interfere in the analysis. To purify the crude extracts to be analysed, several techniques can be used.<\/p>\n<p>This European Standard contains the following extraction and clean-up methods that have been subjected to interlaboratory studies and\/or are adopted throughout Europe:<\/p>\n<ul>\n<li>\n<p>method M: Extraction with acetone and liquid-liquid partition with dichloromethane\/light petroleum, if necessary clean-up on Florisil\u00ae <sup>1)<\/sup> [1], [2], [3];<\/p>\n<\/li>\n<li>\n<p>method N: Extraction with acetone, liquid-liquid partition with dichloromethane or cyclohexane\/ethyl acetate and clean-up with gel permeation and silica gel chromatography [4], [5];<\/p>\n<\/li>\n<li>\n<p>method P: Extraction with ethyl acetate, and if necessary, clean-up by gel permeation chromatography [6].<\/p>\n<\/li>\n<\/ul>\n<p>This European Standard specifies the details of methods M, N and P for the extraction and the clean-up of food samples of plant origin. Several solvents at different volumes are used for extraction. Techniques of clean-up are listed such as liquid-liquid partition, liquid chromatography on various adsorbents and gel permeation chromatography.<\/p>\n<p>A table providing the couples (matrix\/pesticide) which have been submitted to collaborative studies and a list of indicative applicability of the method to different pesticides are given for each method, wherever possible.<\/p>\n<h4>PDF Catalog<\/h4>\n<table border=\"1px\" class=\"des-table\">\n<tr>\n<th>PDF Pages<\/th>\n<th>PDF Title<\/th>\n<\/tr>\n<tr>\n<td><b>4<b><\/td>\n<td>Contents Page  <\/td>\n<\/tr>\n<tr>\n<td><b>5<b><\/td>\n<td>Foreword  <\/td>\n<\/tr>\n<tr>\n<td><b>6<b><\/td>\n<td>Introduction  <\/td>\n<\/tr>\n<tr>\n<td><b>7<b><\/td>\n<td>1 Scope <br \/> 2 Normative references <br \/> 3 Principles  <\/td>\n<\/tr>\n<tr>\n<td><b>8<b><\/td>\n<td>4 General: Summary of procedures <br \/> 4.1 Extraction <br \/> 4.2 Clean-up <br \/> 4.2.1 Liquid-liquid partition <br \/> 4.2.2 Adsorption column chromatography <br \/> 4.2.3 Gel permeation chromatography with Bio-Beads\u00ae S-X31F )  <\/td>\n<\/tr>\n<tr>\n<td><b>9<b><\/td>\n<td>5 Method M: Extraction with acetone and liquid-liquid partition with dichloromethane\/light petroleum, if necessary clean-up on Florisil\u00ae <br \/> 5.1 Principle <br \/> 5.2 Reagents  <\/td>\n<\/tr>\n<tr>\n<td><b>10<b><\/td>\n<td>5.3 Apparatus <br \/> 5.4 Procedure <br \/> 5.4.1 Preparation of the sample <br \/> 5.4.2 Extraction and partition <br \/> 5.4.3 Clean-up <br \/> 5.4.3.1 Method M1  <\/td>\n<\/tr>\n<tr>\n<td><b>11<b><\/td>\n<td>5.4.3.2 Method M2 <br \/> 5.5 Gas chromatography <br \/> 5.6 Calculation of residues  <\/td>\n<\/tr>\n<tr>\n<td><b>12<b><\/td>\n<td>6 Method N: Extraction with acetone, liquid-liquid partition with dichloromethane or cyclohexane\/ethyl acetate, clean-up with gel permeation and silica gel chromatography <br \/> 6.1 Principle <br \/> 6.2 Reagents  <\/td>\n<\/tr>\n<tr>\n<td><b>13<b><\/td>\n<td>6.3 Apparatus  <\/td>\n<\/tr>\n<tr>\n<td><b>14<b><\/td>\n<td>6.4 Procedure <br \/> 6.4.1 Extraction <br \/> 6.4.1.1 General <br \/> 6.4.1.2 Plant material and other foods with a water content exceeding 70 g\/100 g <br \/> 6.4.1.3 Plant material with low water content <br \/> 6.4.2 Partition <br \/> 6.4.2.1 Partition with dichloromethane  <\/td>\n<\/tr>\n<tr>\n<td><b>15<b><\/td>\n<td>6.4.2.2 Partition with cyclohexane\/ethyl acetate <br \/> 6.4.3 Clean-up by gel permeation chromatography <br \/> 6.4.3.1 General <br \/> 6.4.3.2 Packing gel permeation column <br \/> 6.4.3.3 Checking elution volumes <br \/> 6.4.3.4 Clean-up of crude extracts  <\/td>\n<\/tr>\n<tr>\n<td><b>16<b><\/td>\n<td>6.4.4 Additional clean-up for GC determination: Chromatography on a silica gel minicolumn <br \/> 6.4.4.1 Preparation of column <br \/> 6.4.4.2 Checking separation efficiency of silica gel <br \/> 6.4.4.3 Fractionation of sample extract  <\/td>\n<\/tr>\n<tr>\n<td><b>27<b><\/td>\n<td>6.5 Gas chromatography  <\/td>\n<\/tr>\n<tr>\n<td><b>28<b><\/td>\n<td>6.6 Liquid chromatography with tandem-mass spectrometric detection (LC-MS\/MS) <br \/> 6.7 Calculation of residues  <\/td>\n<\/tr>\n<tr>\n<td><b>29<b><\/td>\n<td>6.8 Collaborative studies  <\/td>\n<\/tr>\n<tr>\n<td><b>36<b><\/td>\n<td>6.9 Applicability  <\/td>\n<\/tr>\n<tr>\n<td><b>37<b><\/td>\n<td>7 Method P: Extraction with ethyl acetate, and if necessary, clean-up by gel permeation chromatography <br \/> 7.1 Principle <br \/> 7.2 Reagents <br \/> 7.3 Apparatus  <\/td>\n<\/tr>\n<tr>\n<td><b>38<b><\/td>\n<td>7.4 Procedure <br \/> 7.4.1 Preparation of the sample <br \/> 7.4.2 Extraction <br \/> 7.4.3 Clean-up by gel permeation chromatography (if required) <br \/> 7.4.3.1 Packing gel permeation column <br \/> 7.4.3.2 Checking elution volumes  <\/td>\n<\/tr>\n<tr>\n<td><b>39<b><\/td>\n<td>7.4.3.3 Clean-up of crude extracts <br \/> 7.5 Gas chromatography <br \/> 7.6 Liquid chromatography <br \/> 7.7 Calculation of results  <\/td>\n<\/tr>\n<tr>\n<td><b>40<b><\/td>\n<td>7.8 Proficiency testing data <br \/> 7.9 Applicability  <\/td>\n<\/tr>\n<tr>\n<td><b>43<b><\/td>\n<td>Annex A  (informative)  Average water content of crops and foods  <\/td>\n<\/tr>\n<tr>\n<td><b>44<b><\/td>\n<td>Bibliography  <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p><b>Foods of plant origin. Multiresidue methods for the determination of pesticide residues by GC or LC-MS\/MS &#8211; Methods for extraction and clean-up<\/b><\/p>\n<table class=\"shortdes-table\" style=\"width: 100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Published By<\/td>\n<td>Publication Date<\/td>\n<td>Number of Pages<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/pdfstandards.shop\/product-category\/publishers\/bsi\/\"><b>BSI<\/b><\/a><\/td>\n<td>2013<\/td>\n<td>46<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338080,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[955,2641],"product_tag":[],"class_list":{"0":"post-338074","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-67-080-01","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338074","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338080"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338074"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338074"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338074"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}