{"id":338705,"date":"2024-10-19T23:42:20","date_gmt":"2024-10-19T23:42:20","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-174242020\/"},"modified":"2024-10-25T22:48:38","modified_gmt":"2024-10-25T22:48:38","slug":"bs-en-174242020","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-174242020\/","title":{"rendered":"BS EN 17424:2020"},"content":{"rendered":"

This document describes a procedure for the determination of aflatoxins B1<\/sub>, B2<\/sub>, G1<\/sub> and G2<\/sub> and total aflatoxins (sum of B1<\/sub>, B2<\/sub>, G1<\/sub> and G2<\/sub>) in spices for which EU maximum levels are established, other than paprika, by high performance liquid chromatography (HPLC) with post-column derivatization (PCD) and fluorescence detection (FLD) after immunoaffinity column (IAC) clean-up.<\/p>\n

The method is applicable to the spices capsicum (excluding paprika), pepper, nutmeg, ginger, turmeric and mixtures thereof.<\/p>\n

The method has been validated for aflatoxins B1<\/sub>, B2<\/sub>, G1<\/sub> and G2<\/sub> and total aflatoxins in a range of test samples that comprised: ginger, pepper, nutmeg, chilli, turmeric as individual spices and mixed pepper + chilli + nutmeg (90 + 5 + 5, m<\/i> + m<\/i> + m<\/i>), mixed spice+ginger (6 + 4, m<\/i> + m<\/i>) mixed spice, mixed turmeric+ginger (2 + 8, m<\/i> + m<\/i>).<\/p>\n

The validation was carried out over the following concentration ranges: aflatoxin B1<\/sub> = 1 \u00b5g\/kg to 16 \u00b5g\/kg and total aflatoxins = 2,46 \u00b5g\/kg to 36,1 \u00b5g\/kg.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nundefined <\/td>\n<\/tr>\n
7<\/td>\n1 Scope
2 Normative references
3 Terms and definitions
4 Principle <\/td>\n<\/tr>\n
8<\/td>\n5 Reagents <\/td>\n<\/tr>\n
12<\/td>\n6 Apparatus and equipment <\/td>\n<\/tr>\n
13<\/td>\n7 Procedure
7.1 Extraction of aflatoxins from the sample <\/td>\n<\/tr>\n
14<\/td>\n7.2 Determination of recovery
7.3 IAC clean-up
7.4 HPLC-FLD analysis
7.4.1 General
7.4.2 Confirmation <\/td>\n<\/tr>\n
15<\/td>\n7.4.3 Post-column derivatization
7.4.4 Identification
7.4.5 Calibration
8 Calculation <\/td>\n<\/tr>\n
16<\/td>\n9 Precision
9.1 General
9.2 Repeatability <\/td>\n<\/tr>\n
17<\/td>\n9.3 Reproducibility <\/td>\n<\/tr>\n
18<\/td>\n10 Test report <\/td>\n<\/tr>\n
19<\/td>\nAnnex A (informative)Example conditions for suitable HPLC-FLD systems
A.1 General
A.2 Example HPLC Conditions
A.3 Other examples of suitable columns and mobile phase conditions <\/td>\n<\/tr>\n
21<\/td>\nAnnex B (informative)Typical chromatograms <\/td>\n<\/tr>\n
26<\/td>\nAnnex C (informative)Precision data <\/td>\n<\/tr>\n
29<\/td>\nAnnex D (informative)Supplementary information <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Foodstuffs. Determination of aflatoxins in spices other than paprika by IAC clean-up and HPLC-FLD with post-column derivatization<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2020<\/td>\n34<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338713,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-338705","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338705","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338713"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338705"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338705"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338705"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}