{"id":338422,"date":"2024-10-19T23:40:52","date_gmt":"2024-10-19T23:40:52","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-208132019\/"},"modified":"2024-10-25T22:46:59","modified_gmt":"2024-10-25T22:46:59","slug":"bs-iso-208132019","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-208132019\/","title":{"rendered":"BS ISO 20813:2019"},"content":{"rendered":"

This document specifies minimum requirements of performance characteristics for the detection of nucleic acid sequences (DNA) by molecular methods, such as the polymerase chain reaction (PCR), including different post-PCR detection methods, real-time PCR, single and\/or multiple probe-based detection techniques as well as the combination of such methods.<\/p>\n

The document is applicable to the detection, identification and quantification of DNA from animal species of higher and lower taxonomic groups in foodstuffs, and the validation of applicable methods.<\/p>\n

It is applicable to mammals, birds, reptiles, amphibians, fishes, molluscs, crustaceans and insects. Typical examples for each are listed in Annex A<\/span>.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
8<\/td>\n4 Performance characteristics of the methods
4.1 General
4.2 Scope of the method
4.3 Scientific basis
4.4 Units of measurement
4.5 Applicability <\/td>\n<\/tr>\n
9<\/td>\n4.6 Specificity
4.6.1 General
4.6.2 Requirements for inclusivity testing
4.6.3 Requirements for exclusivity testing <\/td>\n<\/tr>\n
10<\/td>\n4.7 Sensitivity
4.7.1 General
4.7.2 Limit of detection (LOD) <\/td>\n<\/tr>\n
11<\/td>\n4.8 Specific requirements for quantitative methods
4.8.1 General
4.8.2 Limit of quantification (LOQ)
4.8.3 Dynamic range <\/td>\n<\/tr>\n
12<\/td>\n4.8.4 Determination of precision and trueness for quantitative methods
4.9 Robustness
4.9.1 General
4.9.2 Robustness determination by interlaboratory study
4.9.3 Robustness determination by a multifactorial orthogonal test design
5 Single-laboratory validation <\/td>\n<\/tr>\n
13<\/td>\n6 Interlaboratory study (collaborative study)
6.1 General
6.2 Qualitative methods
6.3 Quantitative methods
7 General laboratory and procedural requirements
7.1 General <\/td>\n<\/tr>\n
14<\/td>\n7.2 Facilities, materials and equipment
7.3 Sample preparation and DNA extraction <\/td>\n<\/tr>\n
15<\/td>\n7.4 Use of controls
7.5 Data analysis
7.5.1 Control <\/td>\n<\/tr>\n
16<\/td>\n7.5.2 Conventional PCR
7.5.3 Real-time PCR amplification curves
7.6 Expression of results
7.6.1 Expression of positive results <\/td>\n<\/tr>\n
17<\/td>\n7.6.2 Expression of negative results
7.6.3 Expression of quantitative results
8 Test report <\/td>\n<\/tr>\n
18<\/td>\nAnnex A (informative) List of typical species used for inclusivity and exclusivity testing <\/td>\n<\/tr>\n
23<\/td>\nAnnex B (informative) Examples of unit conversion methods from DNA copy numbers to the ratio of masses <\/td>\n<\/tr>\n
32<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Molecular biomarker analysis. Methods of analysis for the detection and identification of animal species in foods and food products (nucleic acid-based methods). General requirements and definitions<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2019<\/td>\n34<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338426,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-338422","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338422","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338426"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338422"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338422"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338422"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}